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Bio-Techne corporation
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mouse monoclonal immunoglobulin (ig) g antibody against human -sma Becht et al., 2018 )) of the E9.5 (20-23sp) +/+ and Ncx1 −/− PAS scRNA-Seq dataset. Cells were isolated from 4 embryos for each genotype. (G) Percentage of cells in each PAS scRNA-Seq cluster. (H) Bubble plot showing marker genes for each PAS scRNA-Seq cluster. Dot size indicates the percentage of expressing cells; color intensity indicates expression level. (I) Bubble plot showing expression of genes encoding for hematopoietic niche signals in niche cell subsets. Expression is shown separately for +/+ and Ncx1 −/− cells. " width="250" height="auto" />Mouse Monoclonal Immunoglobulin (Ig) G Antibody Against Human Sma, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/mouse monoclonal immunoglobulin (ig) g antibody against human -sma/product/Upstate Biotechnology Inc Average 90 stars, based on 1 article reviews
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human alpha-smooth muscle actin alexa fluor® 488-conjugated antibody Becht et al., 2018 )) of the E9.5 (20-23sp) +/+ and Ncx1 −/− PAS scRNA-Seq dataset. Cells were isolated from 4 embryos for each genotype. (G) Percentage of cells in each PAS scRNA-Seq cluster. (H) Bubble plot showing marker genes for each PAS scRNA-Seq cluster. Dot size indicates the percentage of expressing cells; color intensity indicates expression level. (I) Bubble plot showing expression of genes encoding for hematopoietic niche signals in niche cell subsets. Expression is shown separately for +/+ and Ncx1 −/− cells. " width="250" height="auto" />Human Alpha Smooth Muscle Actin Alexa Fluor® 488 Conjugated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/human alpha-smooth muscle actin alexa fluor® 488-conjugated antibody/product/Bio-Techne corporation Average 94 stars, based on 1 article reviews
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Becht et al., 2018 )) of the E9.5 (20-23sp) +/+ and Ncx1 −/− PAS scRNA-Seq dataset. Cells were isolated from 4 embryos for each genotype. (G) Percentage of cells in each PAS scRNA-Seq cluster. (H) Bubble plot showing marker genes for each PAS scRNA-Seq cluster. Dot size indicates the percentage of expressing cells; color intensity indicates expression level. (I) Bubble plot showing expression of genes encoding for hematopoietic niche signals in niche cell subsets. Expression is shown separately for +/+ and Ncx1 −/− cells. " width="100%" height="100%">
Journal: Cell Reports
Article Title: The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition
doi: 10.1016/j.celrep.2021.110103
Figure Lengend Snippet: Ncx1 −/− embryos display an aberrant peri-aortic microenvironment (A) Confocal WM-IF analysis of E9.5 (22-25sp) +/+ and Ncx1 −/− embryos. Left panels show maximum intensity projections. Boxed area is magnified in the middle and right panels (single 2.5 μm-thick slices). Arrowheads indicate α-SMA + peri-aortic SMCs, absent from Ncx1 −/− embryos (asterisks). Yellow dashed arrow: distance between dorsal aorta (da) and vitelline artery (va). N = 3 ( +/+ ), N = 3 ( Ncx1 −/− ) embryos analyzed. Scale bars: 300 μm (3D), 50 μm (slice). (B) Distance between dorsal aorta and vitelline artery as a measurement of the sub-aortic mesenchyme thickness. Measurements done on images from N = 7 ( +/+ ), N = 5 ( Ncx1 −/− ) different embryos (1-4 images/embryo; 5 measurements / image; 16 ( +/+ ), 17 ( Ncx1 −/− ) different images used. Data are mean ± SD. (C) Flow cytometric analysis of macrophages (Ter119 - CD45 + F4/80 + CD11b + ) in E9.5 (21-25sp) +/+ and Ncx1 −/− caudal part (CP). N = 3 ( +/+ ), N = 3 ( Ncx1 −/− ) embryos were analyzed individually in 2 independent experiments. (D) Quantification of flow cytometric analysis in (C). Data are mean ± SD. (E) Confocal WM-IF of E9.5 (21-24sp) +/+ and Ncx1 −/− embryos (single 2.5 μm-thick slice representative of N = 4 ( +/+ ), N = 4 ( Ncx1 −/− ) embryos). Arrowheads: peri-aortic F4/80 + macrophages. Scale bars: 30 μm. (F) Uniform Manifold Approximation and Projection (UMAP; (
Article Snippet:
Techniques: Isolation, Marker, Expressing
Journal: Cell Reports
Article Title: The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition
doi: 10.1016/j.celrep.2021.110103
Figure Lengend Snippet:
Article Snippet:
Techniques: Blocking Assay, Recombinant, Modification, TaqMan Assay, Expressing, RNA Sequencing Assay, Mutagenesis, Knock-Out, Software